Mouse anti-Human CD50 Antibody

Other species not known

Intercellular adhesion molecule 3 (ICAM-3) (CDw50) (ICAM-R) (CD antigen CD50)

Stimulated human leukocytes were used as the immunogen for this CD50 antibody.

Protein G affinity chromatography

0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Functional Activity (Blocking): Blocks CD50-CD11a interaction
FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Description

This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI). It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.

Storage

Store the CD50 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Expiration:

12 months from the date of shipment when stored properly.

Mouse anti-Human CD50 Antibody

Other species not known

Intercellular adhesion molecule 3 (ICAM-3) (CDw50) (ICAM-R) (CD antigen CD50)

Stimulated human leukocytes were used as the immunogen for this CD50 antibody.

Protein G affinity chromatography

0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Description

Storage

Store the CD50 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Expiration:

12 months from the date of shipment when stored properly.

Mouse anti-Human CD50 Antibody

Size	20 µg, 100 µg
Estimated Lead Time	1-2 weeks
Species	Human
Specificity	Other species not known
Host Species	Mouse
Accession Number	P32942
Gene Id	3385
Gene Symbols	ICAM3
Protein Name / Synonyms	Intercellular adhesion molecule 3 (ICAM-3) (CDw50) (ICAM-R) (CD antigen CD50)
Immunogen	Stimulated human leukocytes were used as the immunogen for this CD50 antibody.
Isotype	Kappa, IgG2b
Purity	Protein G affinity chromatography
Concentration (lot specific)	0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended Applications	Functional Activity (Blocking): Blocks CD50-CD11a interaction FACS: 0.5-1ug/million cells IF: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2)
Domain / Region / Terminus	Cell surface
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	≤-20°C

Size	20 µg, 100 µg
Estimated Lead Time	1-2 weeks
Species	Human
Specificity	Other species not known
Host Species	Mouse
Accession Number	P32942
Gene Id	3385
Gene Symbols	ICAM3
Protein Name / Synonyms	Intercellular adhesion molecule 3 (ICAM-3) (CDw50) (ICAM-R) (CD antigen CD50)
Immunogen	Stimulated human leukocytes were used as the immunogen for this CD50 antibody.
Isotype	Kappa, IgG2b
Purity	Protein G affinity chromatography
Concentration (lot specific)	0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended Applications	Functional Activity (Blocking): Blocks CD50-CD11a interaction FACS: 0.5-1ug/million cells IF: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2)
Domain / Region / Terminus	Cell surface
Research Area	Adaptive Immunity
Shipping Type	Blue ice
Storage	≤-20°C

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Anti-CD50 Antibody