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Mouse Anti-Human HLA B27 [+FITC]

Mouse Anti-Human HLA B27 [+FITC]

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DS-MB-03307
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Antigen Information

Protein Name & Synonyms:
HLA B27
Target Species:
  • Human

Antibody Specifications

Applications:
Flow Cytometry
Concentration:
50 ug/ml
Species Reactivity:
Human

Specificity

This product is a cytotoxic antibody which detects HLA-B27 alloantigen. This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.

Affinity studies by Scatchard analysis showed that this antibody has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).

Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative

Storage/Stability

Store at +4 °C.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Formulation:
Phosphate buffered saline

Preparation

Immunogen:
Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Preservative:
0.09%Sodium Azide
0.2%Bovine Serum Albumin

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.