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Antigen Information
Protein Name & Synonyms:
HLA B27
Target Species:
- Human
Antibody Specifications
Applications:
Flow Cytometry
Concentration:
50 ug/ml
Species Reactivity:
Human
Specificity
This product is a cytotoxic antibody which detects HLA-B27 alloantigen. This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
Affinity studies by Scatchard analysis showed that this antibody has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).
Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative
Affinity studies by Scatchard analysis showed that this antibody has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).
Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative
Storage/Stability
Store at +4 °C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Formulation:
Phosphate buffered saline
Preparation
Immunogen:
Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Preservative:
| 0.09% | Sodium Azide |
| 0.2% | Bovine Serum Albumin |
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
