ELISA (recommended work dilution= For Sandwich-based ELISA and membrane based assay, detecting level is up to 1pg/ml when it is used for capture), Western Blotting (recommended work dilution= 0.1-0.2 µg/ml)
Immunogen was recombinant protein derived from human IL-8. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Supplied as lyophilized purified antibody. It can be reconstituted with sterile PBS before use. Please avoid freeze-thaw cycles.
Interleukin-8, or IL-8, the prototypic member of the C-X-C, or alpha, family of chemokines, is a chemoattractant cytokine involved in the chemotaxis and activation of neutrophils. IL-8 expression has been correlated to a large number of chronic inflammatory diseases, including inflammatory bowel disease (IBD) and atherosclerosis. IL-8 is cleaved from a 99 amino acid precursor to a 72 amino acid, nonglycosylated, and biologically active protein. Whether IL-8 functions in vivo as a monomer or a hydrogen-bonded dimer has not yet been resolved. Two IL-8 receptors, designated IL-8RA and IL-8RB, have been described and share 77% sequence identity. Both are seven-transmembrane domain proteins (7TMD), similar to the G protein-coupled receptors, and, in addition to IL-8, serve as receptors for other members of the alpha and beta chemokine families.
This antibody was selected for its ability to detect human IL-8 in Sandwich ELISA and Array. In ELISA, this antibody shows approximately 5% cross-reactivity with human IL-1b, and no crossreactivity with murine IL-8 and other tested proteins.
Lyophilized: store at 4°C. After reconstitution, if not used within a month, aliquot and store at 20°C. Stability: Two years lyophilized, one month in solution at 4°C.
- Knall, C., Young, S., Nick, J.A., Buhl, A.M., Worthen, G.S., and Johnson, G.L. 1996. Interleukin-8 regulation of the Ras/Raf/mitogen-activated protein kinase pathway in human neutrophils. J. Biol. Chem. 271: 2832-2838.