Mouse anti-Human MUC1 Antibody

Other species not known

Mucin-1 (MUC-1) (Breast carcinoma-associated antigen DF3) (Cancer antigen 15-3) (CA 15-3) (Carcinoma-associated mucin) (Episialin) (H23AG) (Krebs von den Lungen-6) (KL-6) (PEMT) (Peanut-reactive urinary mucin) (PUM) (Polymorphic epithelial mucin) (PEM) (Tumor-associated epithelial membrane antigen) (EMA) (Tumor-associated mucin) (CD antigen CD227) [Cleaved into: Mucin-1 subunit alpha (MUC1-NT) (MUC1-alpha), Mucin-1 subunit beta (MUC1-beta) (MUC1-CT)]

Delipidated extract of human milk fat globule membranes was used as the immunogen for this MUC1 antibody.

Protein G affinity chromatography

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
Prediluted format: incubate for 30 min at RT (2)

Description

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Storage

Store the MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Expiration:

12 months from the date of shipment when stored properly.

Mouse anti-Human MUC1 Antibody

Other species not known

Delipidated extract of human milk fat globule membranes was used as the immunogen for this MUC1 antibody.

Protein G affinity chromatography

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
Prediluted format: incubate for 30 min at RT (2)

Description

Storage

Store the MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Expiration:

12 months from the date of shipment when stored properly.

Mouse anti-Human MUC1 Antibody

Size	20 µg, 100 µg
Estimated Lead Time	1-2 weeks
Species	Human
Specificity	Other species not known
Host Species	Mouse
Accession Number	P15941
Gene Id	4582
Gene Symbols	MUC1, PUM
Protein Name / Synonyms	Mucin-1 (MUC-1) (Breast carcinoma-associated antigen DF3) (Cancer antigen 15-3) (CA 15-3) (Carcinoma-associated mucin) (Episialin) (H23AG) (Krebs von den Lungen-6) (KL-6) (PEMT) (Peanut-reactive urinary mucin) (PUM) (Polymorphic epithelial mucin) (PEM) (Tumor-associated epithelial membrane antigen) (EMA) (Tumor-associated mucin) (CD antigen CD227) [Cleaved into: Mucin-1 subunit alpha (MUC1-NT) (MUC1-alpha), Mucin-1 subunit beta (MUC1-beta) (MUC1-CT)]
Immunogen	Delipidated extract of human milk fat globule membranes was used as the immunogen for this MUC1 antibody.
Isotype	Kappa, IgG2a
Purity	Protein G affinity chromatography
Concentration (lot specific)	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended Applications	Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 0.5-1ug/ml Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT Prediluted format: incubate for 30 min at RT (2)
Domain / Region / Terminus	Cytoplasmic & cell surface
Research Area	Cancer
Shipping Type	Blue ice
Storage	≤-20°C

Size	20 µg, 100 µg
Estimated Lead Time	1-2 weeks
Species	Human
Specificity	Other species not known
Host Species	Mouse
Accession Number	P15941
Gene Id	4582
Gene Symbols	MUC1, PUM
Protein Name / Synonyms	Mucin-1 (MUC-1) (Breast carcinoma-associated antigen DF3) (Cancer antigen 15-3) (CA 15-3) (Carcinoma-associated mucin) (Episialin) (H23AG) (Krebs von den Lungen-6) (KL-6) (PEMT) (Peanut-reactive urinary mucin) (PUM) (Polymorphic epithelial mucin) (PEM) (Tumor-associated epithelial membrane antigen) (EMA) (Tumor-associated mucin) (CD antigen CD227) [Cleaved into: Mucin-1 subunit alpha (MUC1-NT) (MUC1-alpha), Mucin-1 subunit beta (MUC1-beta) (MUC1-CT)]
Immunogen	Delipidated extract of human milk fat globule membranes was used as the immunogen for this MUC1 antibody.
Isotype	Kappa, IgG2a
Purity	Protein G affinity chromatography
Concentration (lot specific)	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended Applications	Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 0.5-1ug/ml Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT Prediluted format: incubate for 30 min at RT (2)
Domain / Region / Terminus	Cytoplasmic & cell surface
Research Area	Cancer
Shipping Type	Blue ice
Storage	≤-20°C

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