This NANOG monoclonal antibody is generated from mouse immunized with NANOG recombinant protein.
Clonality
Monoclonal
Species
Human
Introduction
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).
Specificity
Other Names
NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog
NCBI Accession #
NP_079141.2
Other Accession #
NP_079141.2; XP_002344676.1
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
34620
Recommended Dilutions
WB: 1:100-250; IHC: 1:10-50
Western blot analysis of anti-NANOG monoclonal antibody (Cat# 102-10043) by NONAG recombinant protein. NANOG (NANOG + His tag)(arrow) was detected using the Mab.
NANOG (Cat# 102-10043) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).
NANOG Antibody (Cat# 102-10043)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
