Protein Name & Synonyms:
p53
Applications:
Immunohistology - Frozen, ELISA (dilution: , 1/1000), Immunoprecipitation, Immunohistology - Paraffin, Western Blotting
Concentration (lot specific):
IgG concentration 1.0 mg/ml
Species Reactivity:
Human, This antibody is reported to cross react with bovine, equine and feline Does not react with mouse or rat p53
This product recognizes the human p53 tumour suppressor protein, binding to both wild type and mutant forms. p53 is important in multicellular organisms, where it regulates the cell cycle and thus functions as a tumor suppressor that is involved in preventing cancer.
The antibody recognizes an epitope at the N-terminal end of p53 between amino acids 20-25.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Immunogen:
Recombinant human p53
This product
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Expiration:
12 months from the date of shipment when stored properly.