Mouse Anti-Human PODXL (C-term) (Ascites) Antibody, 0.1 ml
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Product Description
Specifications
| Size | 0.1 mL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Accession Number | O00592 |
| Gene Id | 5420 |
| Gene Symbols | PODXL |
| Protein Name / Synonyms | PODXL (C-term) (Ascites) |
| Clonality | Monoclonal |
| Preparation | This PODXL antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the C-terminal region of human PODXL. |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.
Specificity
Other Names
PODXL; PCLP; PCLP1; Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1
NCBI Accession #
NP_001018121.1;NP_005388.2
Other Accession #
NP_005388.2
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
58635
Recommended Dilutions
IF: 1:100; WB: 1:500-16000
 | Fluorescent confocal image of SY5Y cells stained with PODXL (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-10372 PODXL (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). |
 | PODXL Antibody (C-term)(Ascites)(Cat# 102-10372) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PODXL antibody detected the PODXL protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.