Mouse Anti-Human PRKAA2 Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Accession Number | P54646 |
| Gene Id | 5563 |
| Gene Symbols | PRKAA2 |
| Protein Name / Synonyms | PRKAA2 |
| Clonality | Monoclonal |
| Preparation | This PRKAA2 monoclonal antibody is generated from mouse immunized with PRKAA2 recombinant protein. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Specificity
Other Names
PRKAA2; AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase
NCBI Accession #
NP_006243.2
Other Accession #
NP_006243.2
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
62320
Recommended Dilutions
IF: 1:10-50; WB: 1:100-500
 | Fluorescent image of Hela cell stained with PRKAA2 Antibody(Cat# 102-10116/SG110126AA).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRKAA2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).PRKAA2 immunoreactivity is localized to Cytoplasm significantly. |
 | PRKAA2 antibody (Cat# 102-10116) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PRKAA2 antibody detected the PRKAA2 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.