Product Description

Specifications

Size	20 µg, 100 µg
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Specificity	Other species not known
Host Species	Mouse
Accession Number	O00592
Gene Id	5420
Gene Symbols	PODXL, PCLP, PCLP1
Protein Name / Synonyms	Podocalyxin (GCTM-2 antigen) (Gp200) (Podocalyxin-like protein 1) (PC) (PCLP-1)
Immunogen	A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.
Isotype	IgG1
Purity	Protein G affinity chromatography
Concentration (lot specific)	0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended Applications	FACS: 0.5-1ug/million cells IF: 1-2ug/ml IP: 1-2ug/500ug protein Western Blotting: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2)
Domain / Region / Terminus	Cell surface and cytoplasmic
Shipping Type	Blue ice
Storage	≤-20°C

Description

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.