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Mouse Anti-IgG2a neg. control [+RPE]

Mouse Anti-IgG2a neg. control [+RPE]

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DS-MB-03586
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Antigen Information

Protein Name & Synonyms:
IgG2a
Target Species:
  • Negative Control

Antibody Specifications

Applications:
Flow Cytometry
Reconstitution:
Reconstitute with 1 ml distilled water
Species Reactivity:
Negative Control

Specificity

This product is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells ❏❏ peripheral blood leucocytes, thymocytes, splenocytes and macrophages.

This product recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product. THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at +4 °C.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 1 ml distilled water

Preparation

Immunogen:
Human T lymphocytes
Preservative:
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
This product
Spleen cells from immunised BALB/c mice were fused with cells of the NS-I mouse myeloma cell line.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.