Mouse Anti-MET/HGFR
Mouse Anti-MET/HGFR Antibody, 400 ul
Product Description
Specifications
| Size | 400 µl |
|---|---|
| Protein Name / Synonyms | MET/HGFR |
| Accession Number | P08581 |
| Gene Symbols | MET |
| Gene Id | 4233 |
| Concentration (lot specific) | 0.500 mg/ml |
| Preparation | This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met. |
| Clonality | Monoclonal |
| Species | Human, Mouse |
 | Western blot analysis of lysates from HepG2, H. liver, HT-29 cell line (from left to right), using MET/HGFR Antibody(4AT247. 86. 63). 4AT247. 86. 63 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
 | Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-10006 MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976). |
 | Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent RayBiotech'Â’s different anti-Met monoclonal antibodies that are Cat# 102-10002 through 102-10006. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# 102-10001). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
