Mouse Anti-MET/HGFR Antibody, 400 ul
$334.00
In stock
Code:
102-10006
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Product Description

Specifications

Size 400 µl
Protein Name / Synonyms MET/HGFR
Accession Number P08581
Gene Symbols MET
Gene Id 4233
Concentration (lot specific) 0.500 mg/ml
Preparation This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met.
Clonality Monoclonal
Species Human, Mouse

Introduction

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.

Specificity

Other Names
MET; Hepatocyte growth factor receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; Tyrosine-protein kinase Met
NCBI Accession #
NP_000236.2;NP_001120972.1

Format

Type
Purified Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
155541
Recommended Dilutions
WB: 1:100-500; IF: 1:100
Western blot analysis of lysates from HepG2, H. liver, HT-29 cell line (from left to right), using MET/HGFR Antibody(4AT247. 86. 63). 4AT247. 86. 63 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-10006 MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976).
Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent RayBiotech'’s different anti-Met monoclonal antibodies that are Cat# 102-10002 through 102-10006. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# 102-10001).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
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