ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image) Western Blot: The membrane blot was probed with purified mouse anti-MRSA primary antibody (1:1,000), then with Anti-Mouse IgG secondary antibody conjugated to HRP (1:10,000). The detected protein was clearly visualized by chemiluminescence detection system. Lane 1 and 4. Lysates from two selected MRSA isolated strains, Lane 2-3. Lysates from two selected MSSA strains.
Immunogen was PBP2a recombinant protein derived from MRSA bacteria. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with PBP2a. The IgG fraction of tissue culture supernatant was purified by Protein G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen responsible for serious hospital infections worldwide. These bacteria are resistant to all beta-lactam antibiotics due to the production of an additional penicillin binding protein, the PBP2a (about 75kDa) encoded by the mecA gene, which shows low affinity for this class of antibiotics. According to the resistant and sensitive ability of the bacteria to antibiotics, two main groups are classified: MRSA and MSSA (Methicillinsensitive Staphylococcus aureus).
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
This antibody was selected for its ability to specifically detect PBP2a from MRSA. The antibody has ability to distinguish both MRSA and MSSA in tested assays; in Western Blot analysis, this antibody did not produce a band for any bacterial cell lysates from MSSA. This antibody showed no cross-reactivity with other tested bacterial proteins.
- Zinderman, C. et al. "Community-Acquired Methicillin-Resistant Staphylococcus aureus Among Military Recruits". Emerging Infect Dis. 2004;10(5):941-4.
- Bignardi GE, et al. Detection of the mecA gene and phenotypic detection of resistance in Staphylococcus aureus isolates with borderline or low level methicillin resistance. J Antimicrobiol Chemother. 1996;37:5363.