Product Description

Specifications

Size	20 µg, 100 µg
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Specificity	Other species not tested.
Host Species	Mouse
Accession Number	P19338
Gene Id	4691
Gene Symbols	NCL
Protein Name / Synonyms	Nucleolin (Protein C23)
Immunogen	Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Isotype	IgG1
Purity	Protein G affinity chromatography
Concentration (lot specific)	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended Applications	Flow Cytometry: 0.5-1ug/million cells in 0.1ml IF: 0.5-1ug/ml Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1) Western blot: 0.5-1ug/ml Prediluted format: incubate for 30 min at RT (2)
Domain / Region / Terminus	Nucleoli
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	≤-20°C

Description

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.