This POU5F1 monoclonal antibody is generated from mouse immunized with POU5F1 recombinant protein.
Clonality
Monoclonal
Species
Human, Mouse
Introduction
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Specificity
Other Names
POU5F1; OCT3; OCT4; OTF3; POU domain, class 5, transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3
NCBI Accession #
NP_002692.2
Other Accession #
NP_002692.2
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
38571
Recommended Dilutions
WB: 1:500-4000
POU5F1 Antibody (Cat# 102-10203) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POU5F1 antibody detected the POU5F1 protein (arrow).
POU5F1 Antibody (Cat# 102-10203) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the POU5F1 antibody detected the POU5F1 protein (arrow).
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
