Mouse Anti-SRC Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Accession Number | P12931 |
| Gene Id | 6714 |
| Gene Symbols | SRC |
| Protein Name / Synonyms | SRC |
| Clonality | Monoclonal |
| Preparation | This Src antibody is generated from mouse immunized with a recombinant protein of full length human SRC. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Specificity
Other Names
SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src
NCBI Accession #
NP_005408.1;NP_938033.1
Format
Type
Purified Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
59835
Recommended Dilutions
WB: 1:100-500; IF: 1:10-50
 | Western blot analysis of lysates from HT29, Jurkat cell line (from left to right), using SRC Antibody(Cat# 102-10507). 102-10507 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
 | Fluorescent image of A549 cell stained with SRC Antibody(Cat# 102-10507/SD41014A).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SRC primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).SRC immunoreactivity is localized to Cytoplasm significantly. |
 | The anti-Src Mab (Cat# 102-10507) is used in Western blot to detect Src in Jurkat cell lysate. |
 | FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (102-10507) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 °C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.