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Product Description

Specifications

Size	400 µl
Protein Name / Synonyms	SRC
Accession Number	P12931
Gene Symbols	SRC
Gene Id	6714
Concentration (lot specific)	0.500 mg/ml
Preparation	This Src antibody is generated from mouse immunized with a recombinant protein of full length human SRC.
Clonality	Monoclonal
Species	Human, Mouse

Introduction

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Specificity

Other Names

SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src

NCBI Accession #

NP_005408.1;NP_938033.1

Format

Type

Purified Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

59835

Recommended Dilutions

WB: 1:100-500; IF: 1:10-50

	Western blot analysis of lysates from HT29, Jurkat cell line (from left to right), using SRC Antibody(Cat# 102-10507). 102-10507 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	Fluorescent image of A549 cell stained with SRC Antibody(Cat# 102-10507/SD41014A).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SRC primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).SRC immunoreactivity is localized to Cytoplasm significantly.
	The anti-Src Mab (Cat# 102-10507) is used in Western blot to detect Src in Jurkat cell lysate.
	FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (102-10507) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 ^°C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.