Tumor necrosis factor alpha (TNFalpha), also known as lymphotoxin and cachectin, is a pleiotropic cytokine. TNFalpha is a smaller cytokine with a molecular weight of 26 kDa (transmembrane) and 17 kDa (soluble) that binds to the same receptors producing a vast array of effects similar to those of TNFbeta. Both TNFalpha and TNFbeta share 30% amino acid homology and have similar biological activities. TNFalpha is produced primarily by monocytes, neutrophils and macrophages. It is found in synovial cells and macrophages in the tissues. TNFalpha and TNFbeta can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth. Target tumor cells must express TNF receptors 1 (55 kDa) and 2 (75 kDa) to be killed, with the p55 receptor mediating the cytotoxic response.

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.