Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves

Spiking & Recovery Results
| Sample Type | Average % Recovery | Range (%) |
|---|
| Serum | 125.5 | 110-140 |
| Plasma | 121.8 | 106-137 |
| Cell culture media | 120.4 | 106-133 |
Linearity Results
| Sample Type | Serum | Plasma | Cell Culture Media |
|---|
| 1:2 | Average % of Expected | 117.1 | 120.5 | 122.9 |
| Range (%) | 116-119 | 115-132 | 118-128 |
| 1:4 | Average % of Expected | 113.7 | 122.6 | 117.0 |
| Range (%) | 107-120 | 120-124 | 110-125 |
Kit Components
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 µL volumes
- Adjustable 1-25 µL pipettes for reagent preparation
- 100 µL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 µL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 µL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.