Mouse IL-10 ELISA

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RayBio® Mouse IL-10 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELM-IL10-1
+

Antigen Information

Accession Number:
  • P18893
Gene ID:
  • 16153
Gene Symbols:
  • IL-10
  • IL10
Protein Name & Synonyms:
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Target Species:
  • Mouse

Assay Format

Detection Range:
45 pg/ml - 5000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
45 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Szpak D., Izem L., Verbovetskiy D., et al. ?M?2 Is Antiatherogenic in Female but Not Male Mice. J Immunol. 2018 Apr 1;200(7):2426-2438. doi: 10.4049/jimmunol.1700313.
    Species: Mouse
    Sample type: Conditioned Media
  2. Bil-Lula I., Krzywonos-Zawadzka A., Sawicki G., Wozniak M. An infection of human adenovirus 31 affects the differentiation of preadipocytes into fat cells, its metabolic profile and fat accumulation. J Med Virol. 2016 Mar;88(3):400-7. doi: 10.1002/jmv.24362.
    Species: Mouse
    Sample type: Conditioned Media
  3. Nandi A., Bishayi B. Murine macrophage response from peritoneal cavity requires signals mediated by chemokine receptor CCR-2 during Staphylococcus aureus infection. Immunol Res. 2016 Feb;64(1):213-32. doi: 10.1007/s12026-015-8739-9.
    Species: Mouse
    Sample type: Conditioned Media
  4. Dey P., Ray S., Chaudhuri T. Immunomodulatory activities and phytochemical characterisation of the methanolic extract of Dioscorea alata aerial tuber. Journal of Functional Foods . Volume 23, May 2016, Pages 315-328. https://doi.org/10.1016/j.jff.2016.02.044
    Species: Mouse
    Sample type: Conditioned Media
  5. Dey P., Chaudhuri TK. Immunomodulatory activity of Nerium indicum through inhibition of nitric oxide and cyclooxygenase activity and modulation of TH1/T H2 cytokine balance in murine splenic lymphocytes. Cytotechnology. 2015 Feb 14. [Epub ahead of print]
    Species: Mouse
    Sample type: Conditioned Media
  6. Ghosh C., Bishayi B. Characterization of Toll-Like Receptor-4 (TLR-4) in the Spleen and Thymus of Swiss Albino Mice and Its Modulation in Experimental Endotoxemia. Journal of Immunology Research, Vol 2015 (2015), Article ID 137981 http://dx.doi.org/10.1155/2015/137981
    Species: Mouse
    Sample type: Serum
  7. Ghosh C., Bishayi B. Toll-like receptor 2 and 6 interdependency in the erosive stage of Staphylococcus aureus induced septic arthritis mediated by IFN-? and IL-6--A possible involvement of IL-17 in the progression of the disease. Immunobiology. 2015 Jul;220(7):910-23. doi: 10.1016/j.imbio.2015.01.012
    Species: Mouse
    Sample type: Serum
  8. Ghosh C., Prakash N., Manna S., Bishayi B. Presence of toll like receptor-2 in spleen, lymph node and thymus of Swiss albino mice and its modulation by Staphylococcus aureus and bacterial lipopolysaccharide. Indian J Exp Biol. 2015 Feb;53(2):82-92.
    Species: Mouse
    Sample type: Serum
  9. Roy S., Chaudhuri T. Assessment of Th1 and Th2 cytokine modulatory activity of an edible fern, Diplazium esculentum. Food and Agricultural Immunology Volume 26, Issue 5, 2015 . DOI: 10.1080/09540105.2015.1007449
    Species: Mouse
    Sample type: Serum
  10. Spreadbury I., Ochoa-Cortes F., Ibeakanma C., et al. Concurrent psychological stress and infectious colitis is key to sustaining enhanced peripheral sensory signaling. Neurogastroenterol Motil. 2015 Mar;27(3):347-55. doi: 10.1111/nmo.12497.
    Species: Mouse
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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