Mouse IL-10 ELISA

$398.00
ELM-IL10-1

RayBio® Mouse IL-10 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
45 pg/ml

Looking for higher sensitivity? Check out our new Ultrasensitive Biomarker Testing Service powered by SIMOA™ technology.

Detection Range
45 pg/ml - 5000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-
Species Detected
Mouse
Protein Name & Synonyms
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Accession Number
Gene Names
Il10, Il-10
Gene ID
16153

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • IL10

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Bil-Lula I., Krzywonos-Zawadzka A., Sawicki G., Wozniak M. An infection of human adenovirus 31 affects the differentiation of preadipocytes into fat cells, its metabolic profile and fat accumulation. J Med Virol. 2016 Mar;88(3):400-7. doi: 10.1002/jmv.24362.
    Species: Mouse
    Sample Type: Conditioned Media

  2. Nandi A., Bishayi B. Murine macrophage response from peritoneal cavity requires signals mediated by chemokine receptor CCR-2 during Staphylococcus aureus infection. Immunol Res. 2016 Feb;64(1):213-32. doi: 10.1007/s12026-015-8739-9.

  3. Dey P., Chaudhuri TK. Immunomodulatory activity of Nerium indicum through inhibition of nitric oxide and cyclooxygenase activity and modulation of TH1/T H2 cytokine balance in murine splenic lymphocytes. Cytotechnology. 2015 Feb 14. [Epub ahead of print]
    Species: Mouse
    Sample Type: Conditioned Media

  4. Ghosh C., Bishayi B. Characterization of Toll-Like Receptor-4 (TLR-4) in the Spleen and Thymus of Swiss Albino Mice and Its Modulation in Experimental Endotoxemia. Journal of Immunology Research, Vol 2015 (2015), Article ID 137981 http://dx.doi.org/10.1155/2015/137981
    Species: Mouse
    Sample Type: Conditioned Media

  5. Ghosh C., Bishayi B. Characterization of Toll-Like Receptor-4 (TLR-4) in the Spleen and Thymus of Swiss Albino Mice and Its Modulation in Experimental Endotoxemia. Journal of Immunology Research, Vol 2015 (2015), Article ID 137981 http://dx.doi.org/10.1155/2015/137981
    Species: Mouse
    Sample Type: Serum

  6. Ghosh C., Bishayi B. Toll-like receptor 2 and 6 interdependency in the erosive stage of Staphylococcus aureus induced septic arthritis mediated by IFN-γ and IL-6--A possible involvement of IL-17 in the progression of the disease. Immunobiology. 2015 Jul;220(7):910-23. doi: 10.1016/j.imbio.2015.01.012
    Species: Mouse
    Sample Type: Conditioned Media

  7. Ghosh C., Bishayi B. Toll-like receptor 2 and 6 interdependency in the erosive stage of Staphylococcus aureus induced septic arthritis mediated by IFN-γ and IL-6--A possible involvement of IL-17 in the progression of the disease. Immunobiology. 2015 Jul;220(7):910-23. doi: 10.1016/j.imbio.2015.01.012
    Species: Mouse
    Sample Type: Serum

  8. Ghosh C., Prakash N., Manna S., Bishayi B. Presence of toll like receptor-2 in spleen, lymph node and thymus of Swiss albino mice and its modulation by Staphylococcus aureus and bacterial lipopolysaccharide. Indian J Exp Biol. 2015 Feb;53(2):82-92.
    Species: Mouse
    Sample Type: Serum

  9. Roy S., Chaudhuri T. Assessment of Th1 and Th2 cytokine modulatory activity of an edible fern, Diplazium esculentum. Food and Agricultural Immunology Volume 26, Issue 5, 2015 . DOI: 10.1080/09540105.2015.1007449
    Species: Mouse
    Sample Type: Conditioned Media

  10. Roy S., Chaudhuri T. Assessment of Th1 and Th2 cytokine modulatory activity of an edible fern, Diplazium esculentum. Food and Agricultural Immunology Volume 26, Issue 5, 2015 . DOI: 10.1080/09540105.2015.1007449
    Species: Mouse
    Sample Type: Serum

  11. Spreadbury I., Ochoa-Cortes F., Ibeakanma C., et al. Concurrent psychological stress and infectious colitis is key to sustaining enhanced peripheral sensory signaling. Neurogastroenterol Motil. 2015 Mar;27(3):347-55. doi: 10.1111/nmo.12497.
    Species: Mouse
    Sample Type: Conditioned Media

  12. Ling Z., Liu X., Cheng Y., et al. Clostridium butyricum Combined with Bifidobacterium infantis Probiotic Mixture Restores Fecal Microbiota and Attenuates Systemic Inflammation in Mice with Antibiotic-Associated Diarrhea. Biomed Res Int. 2015; 2015: 582048. Published online 2015 Feb 23. doi: 10.1155/2015/582048
    Species: Mouse
    Sample Type: Serum

  13. Abo Elnazar S., Ghazy A., Ghonelm H., et al. Effect of ultra violet irradiation on the interplay between Th1 and Th2 lymphocytes. Front Pharmacol. 2015; 6: 56. doi: 10.3389/fphar.2015.00056
    Species: Mouse
    Sample Type: Conditioned Media

  14. Singla D., Singla R., Abdelli L., Glass C. Fibroblast growth factor-9 enhances M2 macrophage differentiation and attenuates adverse cardiac remodeling in the infarcted diabetic heart. PLoS One. 2015 Mar 13;10(3):e0120739. doi: 10.1371/journal.pone.0120739
    Species: Mouse
    Sample Type: Tissue Lysate

  15. Ghazy A., Abu El-Nazar S., Ghoneim H., et al. Effect of murine exposure to gamma rays on the interplay between Th1 and Th2 lymphocytes. Front Pharmacol. 2015; 6: 74. doi: 10.3389/fphar.2015.00074
    Species: Mouse
    Sample Type: Conditioned Media

  16. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample Type: Conditioned Media

  17. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample Type: Serum

  18. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample Type: Peritoneal Fluid

  19. Huang Q., Ma X., Zhu D., et al. Total glucosides of peony attenuates experimental autoimmune encephalomyelitis in C57BL/6 mice. J Neuroimmunol. 2015 Jul 15;284:67-73. doi: 10.1016/j.jneuroim.2015.05.009.
    Species: Mouse
    Sample Type: Conditioned Media

  20. Gong J., Liu H., Wu J., et al. Maresin 1 prevents lipopolysaccharide-induced neutrophil survival and accelarates resolution of acute lung injury. Shock. 2015 Oct;44(4):371-80. doi: 10.1097/SHK.0000000000000434.
    Species: Mouse
    Sample Type: BAL Fluid

  21. Choudhury S, Ghosh S., Gupta P., et al. Inflammation-induced ROS generation causes pancreatic cell death through modulation of Nrf2/NF-κB and SAPK/JNK pathway. Free Radic Res. 2015;49(11):1371-83. doi: 10.3109/10715762.2015.1075016
    Species: Mouse
    Sample Type: Serum

  22. Mahanti S., Majhi A., Kundu K., et al. Systemic Staphylococcus aureus infection in restraint stressed mice modulates impaired immune response resulting in improved behavioral activities. J Neuroimmunol. 2015 Nov 15;288:102-13. doi: 10.1016/j.jneuroim.2015.09.003
    Species: Mouse
    Sample Type: Serum

  23. Nandi A., Bishayi B. Host antioxidant enzymes and TLR-2 neutralization modulate intracellular survival of Staphylococcus aureus: Evidence of the effect of redox balance on host pathogen relationship during acute staphylococcal infection. Microb Pathog. 2015 Dec;89:114-27. doi: 10.1016/j.micpath.2015.09.007.
    Species: Mouse
    Sample Type: Conditioned Media

  24. Wang L., Ren L., Tang T., et al. A novel nano-copper-bearing stainless steel with reduced Cu2+ release only inducing transient foreign body reaction via affecting the activity of NF-κB and Caspase 3. Int J Nanomedicine. 2015; 10: 6725–6739.

  25. Ahmad SF., et al. Naringin Attenuates the Development of Carrageenan-InducedAcute Lung Inflammation Through Inhibition of NF-κb, STAT3 and Pro-Inflammatory Mediators and Enhancement of IκBα and Anti-Inflammatory Cytokines. Inflammation, 2014 Online first view August, DOI: 10.1007/s10753-014-9994-y
    Species: Mouse
    Sample Type: Pleural Fluid

  26. Bishayi B., Bandyopadhyay D., Majhi A., Adhikary R. POSSIBLE ROLE OF TOLL LIKE RECEPTOR-2 (TLR-2) IN THE INTRACELLULAR SURVIVAL OF STAPHYLOCOCCUS AUREUS IN MURINE PERITONEAL MACROPHAGES: INVOLVEMENT OF CYTOKINES AND ANTI-OXIDANT ENZYMES. Scandinavian Journal of Immunology. 2014, May Epub Ahead of Print, doi:10.1111/sji.12195
    Species: Mouse
    Sample Type: Conditioned Media

  27. Bishayi B., et al. Effect of exogenous MCP-1 on TLR-2 neutralized murine macrophagesand possible mechanisms of CCR-2/TLR-2 and MCP-1 signallingduring Staphylococcus aureus infection. Immunobiology Ahead of Print 2014, DOI:10.1016/j.imbio.2014.10.013
    Species: Mouse
    Sample Type: Conditioned Media

  28. Dey S., Majhi A., Mahanti S., et al. In Vitro Anti-inflammatory and Immunomodulatory Effects of Ciprofloxacin or Azithromycin in Staphylococcus aureus-Stimulated Murine Macrophages are Beneficial in the Presence of Cytochalasin D. Inflammation, epub ahead of print Nov 2014. DOI: 10.1007/s10753-014-0070-4
    Species: Mouse
    Sample Type: Conditioned Media

  29. Jahromi S., et al. Serum Levels of IL-10, IL-17F and IL-33 in Patients with Asthma: A Case-Control Study. Journal of Asthma Published Ahead of PrintJune 24, 2014. (doi:10.3109/02770903.2014.938353)
    Species: Human
    Sample Type: Serum

  30. Majhi A., Ashikary R., Bhattacharyya A., Mahanti S., Bishayi B. LEVOFLOXACIN AND CEFTRIAXONE IN COMBINATION ATTENUATES LUNG INFLAMMATION IN A MOUSE MODEL OF BACTEREMIC PNEUMONIA BY MULTI-DRUG RESISTANT Streptococcus pneumoniae VIA INHIBITION OF CYTOLYTIC ACTIVITIES OF PNEUMOLYSIN AND AUTOLYSIN. Antimicrob. Agents Chemother. Accepted ahead of Print, June 23 2014. doi:10.1128/AAC.03245-14
    Species: Mouse
    Sample Type: Serum

  31. Meng A., et al.Role of p38 MAPK and STAT3 in lipopolysaccharide‑stimulated mouse alveolar macrophages. EXPERIMENTAL AND THERAPEUTIC MEDICINE 8: 1772-1776, 2014. DOI: 10.3892/etm.2014.2023
    Species: Mouse
    Sample Type: Conditioned Media

  32. Kaur S, Prabha V. Infertility as a Consequence of Spermagglutinating Staphylococcus aureus Colonization in Genital Tract of Female Mice. Sawtell NM, ed. PLoS ONE 2012;7(12):e52325. doi:10.1371/journal.pone.0052325.
    Species: Mouse
    Sample Type: Cervical-Vaginal Fluid

  33. Kaur S, Prabha V. Infertility as a Consequence of Spermagglutinating Staphylococcus aureus Colonization in Genital Tract of Female Mice. Sawtell NM, ed. PLoS ONE 2012;7(12):e52325. doi:10.1371/journal.pone.0052325.
    Species: Mouse
    Sample Type: Tissue Lysate

  34. Savvatis K, van Linthout S, Miteva K, et al. Mesenchymal Stromal Cells but Not Cardiac Fibroblasts Exert Beneficial Systemic Immunomodulatory Effects in Experimental Myocarditis. Alves-Filho JC, ed. PLoS ONE 2012;7(7):e41047. doi:10.1371/journal.pone.0041047.
    Species: Mouse
    Sample Type: Tissue Lysate

Write a review

No posts found

U.S. International

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Cart content Cart content

Customers Also Bought Customers Also Bought