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ISO 13485:2016 cGMP

Mycobacterium Tuberculosis major secretory protein Antigen 85A Recombinant

Catalog #: 228-21183

Product Description

Specifications

Size10 µg, 1000 µg
Estimated Lead Time2-3 weeks
SpeciesBacteria
Accession Number
P0A4V2
Protein Name / Synonyms
Ronectin-binding protein A, Mycolyl transferase 85A.
Expressed Region
ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH.
Expression SystemBaculovirus
Purity
Greater than 90% as determined by SDS-PAGE.
Format
Sterile Filtered colorless solution.
Formulation
The Ag85A solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Shipping TypeBlue ice
Storage-20°C

Description

Description

Recombinant Mycobacterium tuberculosis Ag85A (43-338 a.a) produced in Hi-5 cells is a single, glycosylated polypeptide chain having a molecular mass of 32.8kDa (305 a.a in total).Antigen 85A is fused to a His tag at C-terminus and purified by conventional chromatographic techniques.

Stability & Storage

Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.