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Native ESCHERICHIA COLI BETA GALACTOSIDASE
Catalog #: DS-01-0029
Product Description
Specifications
| Size | 5 mg |
| Estimated Lead Time | 1-2 weeks |
| Species | Bacteria |
| Protein Name / Synonyms | BETA GALACTOSIDASE, |
| Molecular Weight (kDa) | 540 kD |
| Format | Purified protein from Escherichia coli - lyophilized |
| Concentration (lot specific) | Protein content is approximately 20-25% of total weight |
| Preservative | 74% Sucrose |
| Activity | >600 U/mg protein One unit hydrolyses 1 umole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37°,C. |
| Recommended Applications | Functional Assays |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Escherichia coli Beta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Specifications
Size
5 mg
Assay Buffers
Phosphate buffered saline
Stability & Storage
Storage/Stability
Prior to reconstitution store at +4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for12 months from date of reconstitution.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for12 months from date of reconstitution.