| Size | 2 Plate Kit, 4 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Sensitivity | The minimum detectable concentration of MDA is 0.38 uM. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates, Urine |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Inflammation, Oxidative Stress |
| Shipping Type | Blue ice |
| Storage | 2-8°C |
| Storage / Stability | The entire kit should be stored at 2-8°C for up to 6 months from the date of shipment. Do not use the kit beyond its expiration date. For prepared reagent storage, see the manual. |
Nitric oxide (NO) is a highly reactive radical that plays an important role in many essential physiological functions, including neurotransmission, immune response, and apoptosis. It is produced through the oxidation of arginine via nitric oxide synthase, facilitating vital roles in host defense, activation of regulatory proteins, and direct covalent interactions with functional biomolecules. Given its inherent instability, NO undergoes rapid oxidative degradation to form nitrite (NO2-) and nitrate (NO3-). Therefore, quantifying the total concentration of NO2-/ NO3- is a reliable indicator of NO levels.
The RayBio Nitric Oxide (Nitrate/Nitrite) Assay Kit provides an accurate and convenient two-step process for measuring the total nitrate/nitrite concentration in serum, plasma, cell lysates, urine, or other biological fluid samples. First, nitrate is reduced to nitrite. Then, the addition of Griess reagent transforms nitrite into a deep purple azo compound. The nitrite concentration can be accurately determined by measuring the absorbance of the formed azo chromophore using spectrophotometry.
| Component | Size / Description |
|---|---|
| Microplates | Two 96-well plates (12 strips x 8 wells) |
| Sample Buffer | 10 ml |
| Acid Solution | 40 ml |
| Nitrate Standard | 1 vial (100μl) |
| Nitrite Standard | 1 vial (100μl) |
| Griess Reagent I | 1 bottle (powder) |
| Griess Reagent II | 1 bottle (powder) |
| Nitrate Reducing Reagent | 1 bottle (powder) |
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Nitric Oxide (Nitrate/Nitrite) Assay Kit
I used 'RayBiotech's Nitric Oxide (Nitrate/Nitrite) Assay Kit' for measuring nitrate/nitrite content in the neural organoid sample and it worked for human organoids.
Review by Joydeb Majumder, from University of Wisconsin-Madison, on Mar 6, 2025
Nitric Oxide (Nitrate/Nitrite) Assay Kit
