Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information |
|---|
AIF | Cadherin-6 (KCAD) | IFN alpha | IL-1 alpha (IL-1 F1)- Gene Symbol: IL1A
- Gene ID: 443404
- Accession #: Q28579
| IL-1 beta (IL-1 F2)- Gene Symbol: IL1B
- Gene ID: 443539
- Accession #: P21621
|
IL-4- Gene Symbol: IL4 IL-4
- Gene ID: 443327
- Accession #: P30368
| IL-13- Gene Symbol: IL13
- Gene ID: 664707
- Accession #: Q0PW91
| LEKTI (SPINK5)- Gene Symbol: SPINK5
- Accession #: W5P1K5
| LIF- Gene Symbol: LIF
- Accession #: W5P8L4
| RANTES (CCL5)- Gene Symbol: CCL5
- Accession #: W5P4G7
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Ovine Cytokine Array Q2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
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Learn MoreProtocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.