Papain, Lyophilized

Catalog #: 252-10144

Supplied as a lyophilized powder prepared from a 2X crystalline suspension. To insure full activity, the enzyme should be incubated in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes. It is recommend…

Product Description

Specifications

Size	25 mg, 100 mg, 1 gm
Estimated Lead Time	1-2 weeks
Accession Number	P00784
Expression System	Papaya latex
Molecular Weight (kDa)	23.4 kDa (Theoretical)
Format	Lyophilized (powder)
Activity	Activates to at least 15 units per mg protein
Recommended Applications	Cell isolation where it is more gentle than other proteases (i.e.: cortical neurons, retina, and smooth muscle) Protein structural studies, peptide mapping Red cell surface modification for antibody screening or identification Fab preparation from IgG and, Cell isolation where it is more gentle than other proteases (i.e.: cortical neurons, retina, and smooth muscle) Protein structural studies, peptide mapping Red cell surface modification for antibody screening or identification Fab preparation from IgG and IgM antibodies Solubilization of integral membrane proteins Production of glycopeptides from purified proteoglycans Enzymatic wound debridement
Source	Papaya latex
Shipping Type	Ambient temperature
Storage	2-8°C

Description

Other Information

Extinction Coefficient

53,610 cm-1 M-1 (Theoretical) E1%,280 Equals 22.88 (Theoretical)

Isoelectric

8.88 (Theoretical)

CATH

CATHCATHepsin B; Chain A

CAS Registry Number

C.A.S.: 9001-73-4

Storage Conditions

Store at 2-8°C.

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