Introduction
The RayBiotech Phosphotyrosine PD-L1 (B7H1) ELISA Kit is a convenient and sensitive assay to detect Phosphotyrosine PD-L1 (B7H1) in human cell lysate samples
Product Features
- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
Kit Components
- Pre-coated 96-well microplate
- Positive Control
- Anti-phospho Detection Antibody
- HRP-Streptavidin
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Shaker
- Precision pipettes to deliver 2 µL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare the positive control or sample dilutions
Assay Procedure Summary
- Prepare all reagents, samples and positive control as instructed.
- Add 100 µL of positive control and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4°C with gentle shaking.
- Add 100 µL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 µL prepared HRP-Streptavidin to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
