Rabbit Anti-Human PIN1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13526 |
| Gene Id | 5300 |
| Gene Symbols | PIN1 |
| Protein Name / Synonyms | PIN1 (Center) |
| Clonality | Polyclonal |
| Preparation | This PIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-56 amino acids from the Central region of human PIN1. |
| Concentration (lot specific) | 0.423 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PIN1 is an essential nuclear peptidylprolyl cis-trans isomerase (PPIase; EC 5.2.1.8) involved in regulation of mitosis.
Specificity
Predicted Reactivity
Monkey
Other Names
PIN1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; Rotamase Pin1
NCBI Accession #
NP_006212.1
Other Accession #
Q4R383
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
18243
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of PIN1 Antibody (Center) (Cat# 102-18094) in HL-60 cell line lysates (35ug/lane). PIN1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PIN1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | PIN1 Antibody (Center) (Cat# 102-18094) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of PIN1 (arrow) using rabbit polyclonal PIN1 Antibody (Center) (Cat# 102-18094). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIN1 gene. |
 | Confocal immunofluorescent analysis of PIN1 Antibody (Center) (Cat# 102-18094) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.