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Platelet-Derived Growth Factor AB, Human Recombinant
Catalog #: 228-11212
Product Description
Specifications
| Size | 10 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Protein Name / Synonyms | Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AB. |
| Expressed Region | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ile-Glu-Glu-alpha chain and Met-Ser-Leu-Gly-Ser-beta chain. |
| Expression System | E. coli |
| Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from 10mM Acetic Acid. |
| Activity | The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is <, 1 ng/ml, corresponding to a Specific Activity of 1,000,000units/mg. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Platelet-Derived Growth Factor AB Human Recombinant is a heterodimeric, non-glycosylated, polypeptide chain containing 234 amino acids consisting of 13.3kDa alpha-chain and 12.2 beta-chain having a total molecular mass of 25.5kDa. PDGF-AB is purified by proprietary chromatographic techniques.
Stability & Storage
Storage/Stability
Lyophilized Platelet-derived Growth Factor AB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AB should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.