Polyvalent dengue antigen is a 22kDa protein that contains 4 dengue antigen subtypes, produced in E.coli and fused to a 6xHis Tag. The immunoreactive peptide span from the viral envelope to the non-functional region. The polyvalent dengue antigen consists of 25% recombinant antigen from each of the dengue subtypes in their composition. The Polyvalent dengue antigen detects all four dengue subtypes. The sensitivity and specificity could reach approximately 93% and 96% in dengue IgG and IgM ELISA assay.