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Protein A/G Recombinant
Catalog #: 228-11329
Product Description
Specifications
| Size | 10 mg, 100 mg |
| Estimated Lead Time | 2-3 weeks |
| Species | Bacteria |
| Expression System | E. coli |
| Purity | >95% as determined by SDS-PAGE and RP-HPLC. |
| Formulation | Lyophilized white Powder containing no additives. |
| Recommended Applications | Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to, Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5-8. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C2 and C3. The fusion protein has a predicted molecular mass of 50.5 kDa. But it migrates with an apparent molecular mass of approximately 45kDa in SDS-PAGE.
Specifications
Protein Content
A280 of 0.1% solution: ~0.505.
Stability & Storage
Storage/Stability
After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.