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Protein Phosphatase 1, Catalytic Subunit Alpha, Human Recombinant
Catalog #: 228-12316
Product Description
Specifications
| Size | 5 µg, 50 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Accession Number | P62136 |
| Gene Id | 5499 |
| Protein Name / Synonyms | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A, PP1alpha. |
| Expressed Region | MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK. |
| Expression System | E. coli |
| Purity | Greater than 80.0% as determined by SDS-PAGE. |
| Format | Sterile filtered yellowish solution. |
| Formulation | PPP1CA protein solution (0.2mg/ml) containing 50mM Tris-HCl (pH8.5),0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2 and 50% glycerol. , |
| Activity | Specific activity: >,3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate. |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
PPP1CA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 350 amino acids (1-330 a.a) and having a molecular mass of 39.7kDa.PPP1CA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Stability & Storage
Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.