This gene encodes an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:200
Format
Calculated MW
34kDa/44kDa
Observed MW
44kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 27-300 of human ACAA1 (NP_001598.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ACAA1 antibody (144-07422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using ACAA1 antibody (144-07422) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
