The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:100
Format
Calculated MW
98kDa
Observed MW
120kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ACO1 (NP_002188.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ACO1 antibody (144-07867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human prostate using ACO1 antibody (144-07867) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using ACO1 antibody (144-07867) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
