This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Calculated MW
103kDa/131kDa/133kDa/136kDa/140kDa
Observed MW
110kDa, 150kDa
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ADAR (NP_001102.2).
Unmodified
-20°C
Western blot analysis of extracts of various cell lines, using ADAR antibody (144-07869) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat testis using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ADAR antibody (144-07869) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ADAR antibody (144-07869) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
