Adenylate kinase 3 antibody [N1C3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 205 |
| Protein Name / Synonyms | Adenylate kinase 3 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunohistochemistry, Western blot |
| Research Area | Phosphorylation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AK3 antibody, AK3L1 antibody, AK3L2 antibody, MGC166959 antibody, AK4 antibody, adenylate kinase 3-like 1 antibody, ATP-AMP transphosphorylase antibody, mitochondrial adenylate kinase-3 antibody, GTP:AMP phosphotransferase antibody, nucleoside-triphosphate-adenylate kinase antibody, "adenylate kinase isoenzyme 4, mitochondrial antibody", adenylate kinase 4 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
25 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 209 (P27144) of Adenylate kinase 3
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunohistochemistry,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Adenylate kinase 3 antibody [N1C3] detects Adenylate kinase 3 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: Adenylate kinase 3 protein stained by Adenylate kinase 3 antibody [N1C3] (107-10451) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: Adenylate kinase 3 protein stained by Adenylate kinase 3 antibody [N1C3] (107-10451) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded gastric cancer N87 xenograft, using AK3L1(107-10451) antibody at 1:100 dilution.
Sample(30 ug whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: Raji
12% SDS PAGE
107-10451 diluted at 1:1000
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: Raji
12% SDS PAGE
107-10451 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.