ADH4 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse |
| Host Species | Rabbit |
| Accession Number | P08319 |
| Gene Id | 127 |
| Gene Symbols | ADH4 |
| Protein Name / Synonyms | ADH4, ADH-2, HEL-S-4, alcohol dehydrogenase 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
40kDa/42kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human ADH4 (NP_000661.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse liver, using ADH4 antibody (144-05454) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using ADH4 antibody (144-05454) at dilution of 1:200 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.