ADRBK1 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P25098 |
| Gene Id | 156 |
| Gene Symbols | ADRBK1 |
| Protein Name / Synonyms | GRK2, ADRBK1, BARK1, BETA-ARK1, beta-adrenergic receptor kinase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
79kDa
Observed MW
80kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human ADRBK1 (NP_001610.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ADRBK1 antibody (144-01662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)](https://cdn.sanity.io/images/g51potcz/production/62dc29d5c3dd26fcc5444e53b60ca0a22bee5b79-432x350.jpg)
Western blot analysis of extracts from normal (control) and ADRBK1 knockout (KO) 293T cells, using ADRBK1 antibody (144-01662) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)](https://cdn.sanity.io/images/g51potcz/production/2cbe4960f93730583943bfd1286199c05402e6b5-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat brain using ADRBK1 Antibody (144-01662) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)](https://cdn.sanity.io/images/g51potcz/production/bfbbca56918714a0edf7dcee47fc81b396c4a149-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat heart using ADRBK1 Antibody (144-01662) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)](https://cdn.sanity.io/images/g51potcz/production/a3b88fa2f230be592d65d465ed241d3297fef362-350x350.jpg)
Immunohistochemistry of paraffin-embedded human esophageal cancer using ADRBK1 Antibody (144-01662) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)](https://cdn.sanity.io/images/g51potcz/production/a3a53f7e528745bb5e131c9ba81bed7ed7b79d64-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse kidney using ADRBK1 Antibody (144-01662) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (144-01662)](https://www.raybiotech.com/files/images/Antibodies/144/144-01662/6.jpg)
Expiration:
12 months from the date of shipment when stored properly.