Rabbit Anti-AMPK beta (PRKAB1) (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9Y478 |
| Gene Id | 5564 |
| Gene Symbols | PRKAB1 |
| Protein Name / Synonyms | AMPK beta (PRKAB1) (N-term) |
| Clonality | Polyclonal |
| Preparation | This AMPK beta (PRKAB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-34 amino acids from the N-terminal region of human AMPK beta (PRKAB1). |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | AMPK Signaling, Energy Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
Specificity
Predicted Reactivity
Rat
Other Names
PRKAB1; AMPK; 5'-AMP-activated protein kinase subunit beta-1
NCBI Accession #
NP_006244.2
Other Accession #
P80386; NP_006244
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
30382
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | The anti-PRKAB1 Pab (Cat# 102-17098) is used in Western blot to detect PRKAB1 in Jurkat cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2). |
 | Western blot analysis of anti-PRKAB1 Antibody (N-term) (Cat# 102-17098) in mouse heart lysates (35ug/lane). PRKAB1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.