This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 107-386 of human AMT (NP_001158184.1).
Western blot analysis of extracts of various cell lines, using AMT antibody (144-09926) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
12 months from the date of shipment when stored properly.