This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 242-511 of human AMY2A (NP_000690.1).
Western blot analysis of extracts of various cell lines, using AMY2A antibody (144-08045) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver injury using AMY2A antibody (144-08045) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using AMY2A antibody (144-08045) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.