AP2B1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P63010 |
| Gene Id | 163 |
| Gene Symbols | AP2B1 |
| Protein Name / Synonyms | AP2B1, ADTB2, AP105B, AP2-BETA, CLAPB1, AP-2 complex subunit beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
98kDa/104kDa/105kDa
Observed MW
105kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 752-951 of human AP2B1 (NP_001025177.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AP2B1 antibody (144-01995) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human esophagus using AP2B1 Antibody (144-01995) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using AP2B1 Antibody (144-01995) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using AP2B1 Antibody (144-01995) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.