Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q7Z2E3
Gene Id	54840
Gene Symbols	APTX
Protein Name / Synonyms	APTX, AOA, AOA1, AXA1, EAOH, EOAHA, FHA-HIT, aprataxin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Background

This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:20 - 1:50

Format

Calculated MW

5kDa/13kDa/19-40kDa

Observed MW

40kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using APTX antibody (144-05364) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - APTX Polyclonal Antibody (144-05364)

Immunofluorescence analysis of A549 cells using APTX antibody (144-05364).
Immunofluorescence - APTX Polyclonal Antibody (144-05364)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody (144-05364). Western blot was performed from the immunoprecipitate using APTX antibody (144-05364) at a dilition of 1:500.
Immunoprecipitation - APTX Polyclonal Antibody (144-05364)