ARHGDIA Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P52565 |
| Gene Id | 396 |
| Gene Symbols | ARHGDIA |
| Protein Name / Synonyms | ARHGDIA, GDIA1, HEL-S-47e, NPHS8, RHOGDI, RHOGDI-1, rho GDP-dissociation inhibitor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
18kDa/23kDa
Observed MW
25kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ARHGDIA (NP_001172006.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ARHGDIA antibody (144-01214) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (144-01214)](https://cdn.sanity.io/images/g51potcz/production/08dbeb188571d135b74ae7aa94ddeed4e5eb9eb4-486x350.jpg)
Western blot analysis of extracts of various cells, using ARHGDIA antibody (144-01214) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (144-01214)](https://cdn.sanity.io/images/g51potcz/production/c542b4f5f80ea1a9a192da3df7464877edffba32-368x550.jpg)
Western blot analysis of extracts from normal (control) and ARHGDIA knockout (KO) 293T cells, using ARHGDIA antibody (144-01214) at 1:1000 dilution.![Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (144-01214)](https://www.raybiotech.com/files/images/Antibodies/144/144-01214/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.