ARPC3, ARC21, p21-Arc, actin-related protein 2/3 complex subunit 3
Accession Number
O15145
Gene Symbols
ARPC3
Gene Id
10094
Recommended Applications
Western Blotting,IF
Concentration (lot specific)
2.06 mg/ml
Purity
Affinity purification
Background
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:500 - 1:2000 IF 1:50 - 1:100
Format
Calculated MW
20kDa
Observed MW
21kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human ARPC3 (NP_001265485.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ARPC3 Antibody (144-07767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of A549 cells using ARPC3 antibody (144-07767). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
