ARRB1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P49407 |
| Gene Id | 408 |
| Gene Symbols | ARRB1 |
| Protein Name / Synonyms | ARRB1, ARB1, ARR1, beta-arrestin-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:20 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
46kDa/47kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 169-418 of human ARRB1 (NP_004032.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ARRB1 antibody (144-00998) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat liver using ARRB1 antibody (144-00998) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ARRB1 antibody (144-00998) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using ARRB1 antibody (144-00998) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using ARRB1 antibody (144-00998) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ARRB1 antibody (144-00998). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K-562 cells, using 3 ug ARRB1 antibody (144-00998). Western blot was performed from the immunoprecipitate using ARRB1 antibody (144-00998) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.