The protein encoded by this gene is localized to the mitochondrial inner membrane, where it can bind to a highly-related protein, ATAD3A. ATAD3A appears to interact with matrix nucleoid complexes, and the encoded protein negatively regulates that interaction. This gene is expressed almost exclusively in pluripotent embryonic stem cells and some cancer cells. Two transcript variants encoding different isoforms have been found for this gene.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ATAD3B (NP_114127.3).
Western blot analysis of extracts of various cell lines, using ATAD3B antibody (144-08268) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded mouse heart using ATAD3B antibody (144-08268) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using ATAD3B antibody (144-08268) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.