ATP5B Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P06576 |
| Gene Id | 506 |
| Gene Symbols | ATP5B |
| Protein Name / Synonyms | ATP5B, ATPMB, ATPSB, HEL-S-271, ATP synthase subunit beta, mitochondrial |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
56kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B (NP_001677.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ATP5B Antibody (144-05769) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human liver injury using ATP5B antibody (144-05769) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody (144-05769) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody (144-05769) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ATP5B antibody (144-05769) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody (144-05769) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody (144-05769) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ATP5B antibody (144-05769) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ATP5B antibody (144-05769) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using ATP5B antibody (144-05769) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.