ATPIF1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9UII2 |
| Gene Id | 93974 |
| Gene Symbols | ATPIF1 |
| Protein Name / Synonyms | ATPIF1, ATPI, ATPIP, IP, ATPase inhibitory factor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
6kDa/7kDa/12kDa
Observed MW
12kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ATPIF1 antibody (144-05099) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using ATPIF1 antibody (144-05099) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ATPIF1 antibody (144-05099) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human trachea using ATPIF1 antibody (144-05099) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using ATPIF1 antibody (144-05099) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.