ATRIP Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q8WXE1 |
| Gene Id | 84126 |
| Gene Symbols | ATRIP |
| Protein Name / Synonyms | ATRIP, ATR-interacting protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
72kDa/76kDa/83kDa/85kDa
Observed MW
86kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ATRIP (NP_569055.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ATRIP antibody (144-07139) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ATRIP antibody (144-07139). Western blot was performed from the immunoprecipitate using ATRIP antibody (144-07139) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.