B9D1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UPM9 |
| Gene Id | 27077 |
| Gene Symbols | B9D1 |
| Protein Name / Synonyms | B9D1, B9, EPPB9, JBTS27, MKS9, MKSR1, B9 domain containing 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a B9 domain-containing protein, one of several that are involved in ciliogenesis. Alterations in expression of this gene have been found in a family with Meckel syndrome. Meckel syndrome has been associated with at least six different genes. This gene is located within the Smith-Magenis syndrome region on chromosome 17.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
16kDa/22kDa
Observed MW
23kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human B9D1 (NP_056496.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using B9D1 antibody (144-07973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using B9D1 antibody (144-07973) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using B9D1 antibody (144-07973) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using B9D1 antibody (144-07973) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.