[KO Validated] BAZ1B Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UIG0 |
| Gene Id | 9031 |
| Gene Symbols | BAZ1B |
| Protein Name / Synonyms | BAZ1B, WBSCR10, WBSCR9, WSTF, tyrosine-protein kinase BAZ1B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
170kDa
Observed MW
190kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BAZ1B (NP_115784.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BAZ1B antibody (144-09851) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)](https://cdn.sanity.io/images/g51potcz/production/d72fcaf100b25882fde267f8367c1bad6af82c88-645x350.jpg)
Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody (144-09851) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)](https://cdn.sanity.io/images/g51potcz/production/126f2e35dd872ee547635bdb5c9742a3aa6c74e4-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)](https://cdn.sanity.io/images/g51potcz/production/c5c76fe5c838b46d5b40f3c7d15ba64acd5ed040-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)](https://cdn.sanity.io/images/g51potcz/production/215ef2c36bb116afad90833e7d475c913e7e09d5-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)](https://cdn.sanity.io/images/g51potcz/production/c54f9a40abb5c563637c9a116a4deb0738628535-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)](https://cdn.sanity.io/images/g51potcz/production/d480ad2b7a6e15537ecea9ecdc79d16097255949-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)](https://www.raybiotech.com/files/images/Antibodies/144/144-09851/7.jpg)
Immunofluorescence analysis of HeLa cells using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)](https://www.raybiotech.com/files/images/Antibodies/144/144-09851/8.jpg)
Expiration:
12 months from the date of shipment when stored properly.