Rabbit Anti-Bid (BH3 Domain Specific) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P55957 |
| Gene Id | 637 |
| Gene Symbols | BID |
| Protein Name / Synonyms | Bid (BH3 Domain Specific) |
| Clonality | Polyclonal |
| Preparation | This Bid antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-103 amino acids from human Bid. |
| Concentration (lot specific) | 1.855 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.
Specificity
Other Names
BID; BH3-interacting domain death agonist; p22 BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID
NCBI Accession #
NP_001187.1;NP_001231496.1;NP_001231498.1;NP_001231499.1;NP_001231501.1;NP_932070.1;NP_932071.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
21995
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | The anti-Bid BH3 domain Pab (Cat# 102-11864) is used in Western blot to detect Bid BH3 in HL-60 cell lysate. |
 | Western blot analysis of anti-hBid-BH3 Pab (Cat# 102-11864) in mouse lung tissue lysates (35ug/lane). hBid-BH3(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Bid BH3 Domain Antibody (Cat# 102-11864), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3) (Cat# 102-11864). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene. |
 | Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3) (Cat# 102-11864). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.